Size sensing and dynamic evaluation of reactions with acoustic-gravitational field

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K13722
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Analytical chemistry
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Okada Tetsuo 東京工業大学, 理学院, 教授 (20183030)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 超音波 / マイクロ粒子 / 金ナノ粒子 / 密度 / 微量計測

Outline of Final Research Achievements

This work proposes a concept for sensing based on the density change of microparticles (MPs) caused by a biochemical reaction. The MPs are levitated by a combined acoustic-gravitational force at the position determined by the density and compressibility. When gold nanoparticles (AuNPs) are bound on the surface of polymer MPs through a reaction, the density of the MPs dramatically increases, and their levitation position in the acoustic-gravitational field is lowered. Because the shift of the levitation position is proportional to the number of AuNPs bound on one MP, we can determine the number of molecules involved in the reaction. The avidin-biotin reaction is used to demonstrate the effectiveness of this concept. The number of molecules involved in the reaction is in a zmol range; thus, the method has potential for highly sensitive detection.

Free Research Field

分析化学