Construction of multi-functional protein materials for artificial blood vessels

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K13781
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Polymer/Textile materials
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Kobatake Eiry 東京工業大学, 生命理工学院, 教授 (00225484)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 多機能タンパク質 / 人工血管 / 生体融和材料 / 疎水性材料 / 細胞接着

Outline of Final Research Achievements

We constructed a novel multi-functional protein which can assemble on a hydrophobic surface with high density and high orientation. A structural unit with hydrophobic property was combined with functional unit with cell adhesive activity. The designed protein was expressed in E.coli and purified with affinity chromatography. The resulting protein was assmbled well on the hydrophobic polymer which was generally used for the artifical blood vessels. Furthermore, the surface of the protein-assembeld hydrophobic polymer showed well cell adhesive property.
The newly constructed protein in this study would be a useful material for the artifical blood vessels.

Free Research Field

タンパク質工学