On Chip Purifyication of hiPSC-derived Cells Using a Fishnet-like Mircofluidics

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K13911
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Intelligent mechanics/Mechanical systems
• Research Institution: Osaka University (2017); Kyoto University (2015-2016)
• Principal Investigator: Liu Li 大阪大学, 医学系研究科, 特任准教授(常勤) (50380093)
• Co-Investigator(Kenkyū-buntansha): 南 一成 大阪大学, 医学系研究科, 特任准教授(常勤) (40362537)
• Co-Investigator(Renkei-kenkyūsha): KOTERA Hidetoshi 京都大学, 工学研究科, 教授 (20252471)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 幹細胞 / マイクロ加工 / ナノファイバー / 組織 / 細胞純化

Outline of Final Research Achievements

Human induced pluripotent stem cells (hiPSC) can be differentiated at high efficiency into cells of a targeting type but the resulted cell population has to be of high purity to avoid teratomas for clinical therapies. Herein we report a microfluidic device with integrated and surface functionalised fishnet-like structures for specific cell capture. With the help of a flow derivation surface pattern, cells in solution are forced to cross the fishnet-like structure, resulting in high efficiency and selective retention of a chosen cell population. A suspension of hiPSCs spiked in culture medium or hiPSC derived cardiomyocytes (CMs) containing medium was used for devices function validation. We found a hiPSC capture rate as high as 80% and a remarkable increase of the CM population rate in the recovered suspension without affecting the cell viability.

Free Research Field

幹細胞、組織工学