Design of immune tolerance inducing peptide using the peptide array analysis technique

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14230
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: OKOCHI Mina 東京工業大学, 物質理工学院, 教授 (70313301)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: ペプチド / アレルギー / バイオチップ

Outline of Final Research Achievements

Peptide microarray is useful for the antibody epitope mapping since it enables high-throughput binding interaction analysis of medical serological samples. In this study, milk allergy mouse model was made by four times immunization of casein with alum adjuvant and heterovalent IgE epitope was analyzed using the milk peptide microarray that composed peptide library of six milk protein amino acid sequences. Using the milk allergy mouse sera-IgE sensitized basophilic leukemia cells, design of short epitope peptides was investigated for inhibition of allergic reaction by allergen addition. When the short epitope peptides was administrated to milk allergy mouse, allergic responses such as scratching and rectal temperature decrease were suppressed after allergen administration. Therefore, it was indicated that the short epitope peptides could be used as the personalized medicine in oral immunotherapy and it would help safe administration of allergens by suppressing allergic reactions.

Free Research Field

生物機能・バイオプロセス