Development of genetically encoded protein synthesis activity reporter

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14311
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: The University of Tokyo
• Principal Investigator: Ikeuchi Yoshiho 東京大学, 生産技術研究所, 講師 (30740097)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: タンパク質合成 / 翻訳 / リボソーム / 神経 / 形態

Outline of Final Research Achievements

In this study, we developed a reporter protein for cellular protein synthesis that is altered upon neural plasticity. To directly represent the cellular protein synthesis activity at the time of measurement, we designed an artificial reporter protein that is only fluorescent during it is under synthesis, and lose fluorescence after protein synthesis by self digestion. We generated the construct, expressed the proteins, and checked the self digestion ability of the reporter. We optimized the construct to maximize the self digestion ability.

Free Research Field

分子神経科学