Molecular basis for the establishment of early metastatic lesion through cell adaptation

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14381
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: Kobe University
• Principal Investigator: Shimono Yohei 神戸大学, 医学研究科, 准教授 (90594630)
• Research Collaborator: MINAMI Hironobu 神戸大学, 大学院医学研究科, 教授 ; TAKAO Shintaro 神戸大学, 大学院医学研究科, 教授 ; CLARKE Michael スタンフォード大学, 幹細胞医学研究所, 教授 ; ISOBE Taichi スタンフォード大学, 幹細胞医学研究所, 博士研究員 ; MUKOHYAMA Junko 神戸大学, 大学院医学研究科, 大学院生
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 乳がん / 肝転移 / 異種移植マウス / マイクロRNA

Outline of Final Research Achievements

Successful engraftment of disseminated metastatic cancer cells will require an adaptation of cancer cells to the specific microenvironments of metastasized organs. We identified the profile of microRNAs and genes specifically expressed in the early metastatic cancer cells directly isolated from the metastatic organ of human breast cancer patient-derived xenograft mice. We then analyzed the regulatory mechanism for the expression of miR-106b-25 cluster miRNAs. We also established the in vitro culture system in which primary liver cells and human breast cancer patient-derived xenograft cells were co-cultured to facilitate analyses of the adaptation of breast cancer cells to metastasized organs.

Free Research Field

腫瘍学