Regulation epithelial stem cells by reactive oxgens species during oncogenic transformation

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14390
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: National Cancer Center Japan
• Principal Investigator: Okamoto Koji 国立研究開発法人国立がん研究センター, 研究所, 分野長 (80342913)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 癌

Outline of Final Research Achievements

In general, reactive oxygen species (ROS) inhibit proliferation of non-tumor cells. Recently we found that high levels of ROS function to promote proliferation of colon cancer stem cells in vitro. We aimed to elucidate the molecular mechanism by which ROS differentially affect cell proliferation during carcinogenesis. In order to investigate the regulation of ROS levels during colon carcinogenesis, we established the in vitro three-dimensional culture from non-tumor and tumor tissues, and examined the gene expression profiles as well as ROS levels. We revealed that levels of NADPH oxidase increased during the carcinogenesis. Detailed investigation based on these observation is underway.

Free Research Field

分子腫瘍学