Structural elucidation of dioxin recognition by aryl hydrocarbon receptor

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14471
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: Ohto Umeharu 東京大学, 大学院薬学系研究科(薬学部), 准教授 (90451856)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: ダイオキシン / 環境ホルモン

Outline of Final Research Achievements

We conducted the structural biology of aryl hydrocarbon receptor (AhR), known as dioxin receptor. We tried to prepare the pre-nuclear translocation complex of AhR but it was not successful. We conducted the crystallization of the AhR inhibitory complex, AhRR/ARNT complex, and determined its crystal structure. To obtain the structural information of the AhR transcriptional activation complex, AhR/ARNT complex, we tried to prepare AhR/ARNT complex derived from various organism species, and expressed and purified various mutants of AhR/ARNT.

Free Research Field

構造生物学