Development of a method to determine hydroxymethylcytosine in DNA at single base resolution utilizing recombinant DNMT1

2015 Fiscal Year Final Research Report

• Project/Area Number: 15K14478
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Osaka University
• Principal Investigator: TAJIMA Shoji 大阪大学, たんぱく質研究所, 教授 (50132931)
• Co-Investigator(Kenkyū-buntansha): KIMURA Hironobu 大阪大学, 蛋白質研究所, 助教 (60378891) ; SUETAKE Isao 大阪大学, 蛋白質研究所, 准教授 (80304054)
• Co-Investigator(Renkei-kenkyūsha): ITO Takashi 九州大学, 大学院医学研究院, 教授 (90201326)
• Project Period (FY): 2015-04-01 – 2016-03-31
• Keywords: DNAヒドロキシメチル化修飾 / DNAメチル化 / DNAメチル基転移酵素

Outline of Final Research Achievements

Genomic DNA methylation modification plays crucial role in development. Major players of the establishment of DNA methylation and replication-coupled maintenance DNA methylation have been identified. Recently, active DNA demethylation step has been reported to be catalyzed initially by TET family oxidases to produce hydroxymethylcytosine. Although several techniques to identify the position of hydroxymethylcytosine in genome have been reported, they possess disadvantages. In the present study, we have developed a new technique to determine hydroxymethylcytosine at single base resolution utilizing recombinant DNA methyltransferase DNMT1.

Free Research Field

DNAメチル化の生化学