Construction of a new reconstruction system and analysis of dynamic protein secretion machinery

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14490
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: Yoshikane Kunihiro 奈良先端科学技術大学院大学, バイオサイエンス研究科, 研究員 (20467616)
• Co-Investigator(Renkei-kenkyūsha): TSUKAZAKI Tomoya 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (80436716) ; KONNO Hiroki 金沢大学, バイオAFM先端研究センター, 准教授 (80419267)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 蛋白質

Outline of Final Research Achievements

Protein secretion machinery transports precursor proteins across membranes with dynamic structural changes. Although the detailed structures of each factor have been reported by X-ray crystallography, it is still unknown how the factors undergo conformational transitions. In this study, we established a new reconstruction system using nanodiscs and observed the nanodiscs by high speed AFM. It is time to analyze the protein secretion using this tool.

Free Research Field

蛋白質科学