2016 Fiscal Year Final Research Report
Development of a new freeze replica method to observe the hydrophobic portion of membrane lipids
| Project/Area Number |
15K14506
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Research Collaborator |
MAKOTO ARITA 独立行政法人理化学研究所, 総合生命医科学研究センター, チームリーダー (80292952)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 膜脂質 / 電子顕微鏡 |
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| Outline of Final Research Achievements |
Phospholipids constitute the basic framework of biological membranes and they are known to contain highly variable acyl chains in their hydrophobic tail portion. However, it is hardly known how those acyl chains distribute in the cellular membrane. In this project, we developed a method to define the nano-scale distribution of a specific kind of acyl chain by utilizing alkyne-labeled fatty acids and quick-freezing freeze-fracture electron microscopy. By performing click reaction on the freeze-fracture replica, labeling for the acyl chain was observed in cellular membranes, and moreover, it was shown to form clusteres in a limited region of the membrane.
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| Free Research Field |
細胞生物学
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