Single molecule imaging of endogenous protein in living cells using spontaneously blinking fluorophore

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14516
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Kambara Taketoshi 国立研究開発法人理化学研究所, 生命システム研究センター, 研究員 (40451637)
• Co-Investigator(Renkei-kenkyūsha): OKADA Yasushi 国立研究開発法人理化学研究所, 生命システム研究センター, チームリーダー (50272430) ; URANO Yasuteru 東京大学, 薬学系, 教授 (20292956) ; KAMIYA Mako 東京大学, 医学系, 助教 (90596462)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: １分子イメージング

Outline of Final Research Achievements

It is expected that protein dynamics and protein-protein interaction in the crowded cellular environments are different from those in vitro. However, direct evaluation of the effect of macromolecular crowing was difficult, since the method to observe freely diffusing single molecule in the cytoplasm has not been developed. In this study, we performed single molecule imaging of freely diffusing endogenous protein in living cells using spontaneously blinking fluorophore.

Free Research Field

生物物理