Signaling induced by calcium depletion from the endoplasmic reticulum: regulation mechanisms and its roles in myoblast differentiation

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K14518
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: MORISHIMA Nobuhiro 国立研究開発法人理化学研究所, 伊藤ナノ医工学研究室, 特別嘱託職員 (40182232)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 小胞体 / カルシウム枯渇 / 細胞内シグナル伝達 / 筋芽細胞

Outline of Final Research Achievements

Endoplasmic reticulum (ER) is the calcium store in cells. In response to various stimuli, the ER releases calcium into the cytosol where calcium dependent proteins are activated. On the other side of the coin, calcium depletion from the ER occurs. We have previously found that ER calcium depletion is critical for skeletal myoblast differentiation. In the present project, I have examined the precise timing of ER calcium depletion in differentiating myoblast cells. By analyzing the behaviors of proteins that regulate calcium dynamics in the ER, and those of calcium dependent proteins, I have gotten clues as to the regulation mechanism of ER calcium depletion and its roles in skeletal muscle differentiation.

Free Research Field

細胞生物学