Development and application of methods for identifying protein-DNA interactions in a tissue-specific manner

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K14527
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Kochi University of Technology
• Principal Investigator: Kamachi Yusuke 高知工科大学, 環境理工学群, 教授 (90263334)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 転写因子 / クロマチン免疫沈降 / エピトープタグ

Outline of Final Research Achievements

Analysis of the interaction between protein and genomic DNA in cells is important for understanding gene regulatory mechanism. A technique called chromatin immunoprecipitation is used for this purpose, but there are various technical limitations such as the requirement of high quality antibodies. This research aims to develop a versatile method that detects protein-DNA interactions in specific tissues of animal embryos with high sensitivity by introducing a specific short amino acid sequence such as a biotin tag to the target protein.

Free Research Field

分子発生生物学