2016 Fiscal Year Final Research Report
Investigation roles of the miRNA pathway in maternal-to-xygotic transition
Project/Area Number |
15K14530
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Developmental biology
|
Research Institution | Kumamoto University |
Principal Investigator |
Nakamura Akira 熊本大学, 発生医学研究所, 教授 (90323245)
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Keywords | ショウジョウバエ / CRISPR-Cas9 / miRNA / ゲノム編集 |
Outline of Final Research Achievements |
The maternal-to-Zygotic transition (MZT) involves degradation of maternal product to switch zygotic gene-oriented development, and is the conserved process in animal development. To investigate roles of the miRNA pathway genes in MZT in the Drosophila embryo, we generated several GFP knocked-in flies for Dcr-1, Ago1 and Gw. We knocked down these GFP fusion protein specilicaally in early embryogenesis by activating deGradFP system, which poly-ubiquitinates GFP-fusion proteins to promote their degradation. We found that when GFP-tagged Gw was knocked down, about 50% of embryos stalled embryogenesis at the stage during which the MZT starts, suggesting that the miRNA pathway is indeed involved in the MZT.
|
Free Research Field |
発生生物学
|