Development of rice with enhanced nitrogen acquisition ability from soil

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K14627
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Science in genetics and breeding
• Research Institution: Ishikawa Prefectural University
• Principal Investigator: Hamada Tatsuro 石川県立大学, 生物資源環境学部, 准教授 (50310496)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: イネ / アスパラギン酸プロテアーゼ / タンパク質 / 窒素源 / 形質転換体 / 根圏

Outline of Final Research Achievements

Transgenic rice overexpressing aspartic protease gene (OsASP2) and nontransformed rice were cultivated in MS and MS-IN, casein media for 30 days, and the growth of both were compared. In MS medium, no significant growth difference was found between them. On the other hand, in MS-IN, casein medium, leaf length and dry weight of the OsASP2 transgenic rice were about 3.7 and 1.5 times those of the non-transformant, respectively. This result revealed that the release of aspartic protease from the root to the rhizosphere in the transgenic rice contributed to the use of protein as a nitrogen source for growth.

Free Research Field

植物生理学