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2017 Fiscal Year Final Research Report

Analysis of Cold-Adaptation Mechanism of Food Spoilage Bacteria and Its Application to Food Industry

Research Project

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Project/Area Number 15K14732
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Food science
Research InstitutionKyoto University

Principal Investigator

KURIHARA Tatsuo  京都大学, 化学研究所, 教授 (70243087)

Co-Investigator(Renkei-kenkyūsha) KAWAMOTO Jun  京都大学, 化学研究所, 助教 (90511238)
Project Period (FY) 2015-04-01 – 2018-03-31
Keywords低温増殖性細菌 / 食品変敗 / 低温適応機構 / 表層タンパク質 / タンパク質生産
Outline of Final Research Achievements

We found that Leuconostoc mesenteroides NH04 isolated from spoiled processed meat products has higher adhesion to mucin than the ATCC8292 strain and the NBRC3832 strain of the same species, and its adhesiveness is increased in the presence of sodium nitrite used as a food additive. A homolog of bifunctional acetaldehyde-CoA/alcohol dehydrogenase was identified as a cell-surface protein of NH04 possibly involved in its adhesion. We also identified homologs of proteins involved in RNA modification/folding and transport of compatible solutes as cold-inducible proteins of NH04 that may be involved in its cold adaptation.

Free Research Field

分子微生物科学

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Published: 2019-03-29  

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