Microenvironment in mine iducing biodiversity of leaf-miner and physiological interaction between tree and insect

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14759
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Forest science
• Research Institution: Kyoto Prefectural University
• Principal Investigator: Ikeda Takefumi 京都府立大学, 生命環境科学研究科, 教授 (50183158)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: リーフマイナー / 葉の組織・解剖 / 光合成活性 / 非破壊検査

Outline of Final Research Achievements

Histology, anatomy and physiology in lear-miner attacked foliage. Leaf-miner is an endophagous insect infiltrating into live foliage and making a micro-cavity as a mine following feeding on mesophyll cells. <Anatomy> mine without leaf browning: no change of cell activity. mine with leaf browning: defensive reaction such as deposition of tannin, subprime and lignin as chemicals. Non browning leaf; no remarkable denaturation and necrosis of cells. decrease in Fv/Fm --> decrease in photosynthetic activity. remarkable decrease in Fm --> inhibition of oxygen transmission system PSII. Browning leaf; remarkable decrease in Fv/Fm --> palisade cells were fed. Decrease in Fo and Fm in mine part of leaf --> empty space of mine reduce light intensity reaching mesophyll cells. Mine is a separate space from outside and stable environment for leaf-miner. Gas concentration of air in mine might depend on a cuticle gas permeability.

Free Research Field

樹木生理学