Generation of transgenic Medaka producing a transporter targeting to fish oocytes

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K14797
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Aquatic life science
• Research Institution: Hokkaido University
• Principal Investigator: HIRAMATSU Naoshi 北海道大学, 水産科学研究院, 准教授 (10443920)
• Co-Investigator(Renkei-kenkyūsha): IJIRI Shigeho 北海道大学, 大学院水産科学研究院, 准教授 (90425421) ; KAWAKAMI Koichi 国立遺伝学研究所, 教授 (70195048)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 分子輸送 / バイオリアクター / 卵 / ビテロジェニン / メダカ / 生物工学

Outline of Final Research Achievements

The aim of this study is to obtain the first technical basis for the development of a bio-reactor system through the production of model transporters in transgenic fish, with the final goal of which constructs a system transporting various effective materials across generations (i.e., from mother to egg or larva) in fish. Various expression constructs were generated by combining transporters, fluorescent proteins, a linker, trans-genic vector systems. Trans-genic medaka expressing fluorescent model transporters, as well as a model transporter with a linker, were produced as the second (F1) and the third (F2, the fluorescent transporter alone) trans-genic generations. This study provided fundamental knowledge for the next examination toward a massive bio-production of the transporters.

Free Research Field

魚類繁殖生理学