Time series analysis of unknown differentiation mechanisms and related genes for hair follicle generation

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K14866
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Nishifuji Koji 東京農工大学, (連合)農学研究科(研究院), 准教授 (20365422)
• Co-Investigator(Kenkyū-buntansha): 井手 香織 東京農工大学, (連合)農学研究科(研究院), 講師 (40550281) ; 角田 茂 東京大学, 医科学研究所, 准教授 (80345032)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 毛包

Outline of Final Research Achievements

In this study, we investigated whether nestin-expressing neural stem cells and their descendants can be mixed in keratin 15-expressing hair follicle bulge stem cells. We also aimed to generate plasmid constructs to investigate the fate of epithelial-neural stem cells. In murine anagen and hair follicle bulge, keratin 15-expressing cells with features of nestin-expressing cells or their descendants could not be detected by immunofluorescence analysis. Meanwhile, we could successfully generated plusmid constructs that could express FlpERT2, which induces Flp-FRT site specific recombination under the presence of tamoxifen, by Cre-loxP recombination in keratin 15 and nestin co-expressing cells.

Free Research Field

獣医学