Foot and mouth disease virus specific translation inhibition and application for development of disease resistant livestock

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14871
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Kagoshima University
• Principal Investigator: Kohara Kyoko 鹿児島大学, 農水産獣医学域獣医学系, 教授 (20225478)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: FMDV / IRES / 宿主因子 / PTB / 4E-BP1 / ITAF45 / 細胞株

Outline of Final Research Achievements

FMD is a highly contagious infectious disease in cloven-hoofed animals. FMDV possess a positive sense, single stranded RNA genome. Internal ribosomal entry site (IRES) element exists within its 5′ UTR of the viral RNA. IRES mediated translation is unique translation mechanism, which are mostly observed in picornaviruses and hepacivirus. Our data indicated that IRES-mediated translational activity was not linked to FMDV host range. ITAF45 promoted IRES-mediated translation in all cell lines, and the effects of poly-pyrimidine tract binding protein (PTB) and eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) were observed only in FMDV-susceptible cells. Thus, PTB and 4E-BP1 may influence the host range of FMDV. Moreover, we have established the cell line expressing FMDV-IRES reporter gene, which can be applicable for the screening of FMDV-IRES inhibitors. Thus, these findings may contribute the development of disease resistant domestic animals and novel drugs.

Free Research Field

ウイルス学、分子生物学