The effective method for generation of platelets from canine iPS cells by perfusion cocultivation

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14888
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Integrative animal science
• Research Institution: Osaka Prefecture University
• Principal Investigator: INABA TOSHIO 大阪府立大学, 生命環境科学研究科, 教授 (00137241)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 再生医療 / 統合動物科学 / ｉＰＳ細胞 / 血小板 / バイオテクノロジー / トランスレーショナルリサーチ

Outline of Final Research Achievements

The objective of this study was in the development of the effective method for generation of platelets from canine iPS cells, and the following results were obtained. We generated canine hematopoietic cytokine-secreting feeder cells by transfection of the genes to OP9 cells. While we established a stable and long-term culture of canine iPS cells, we could not differentiate them into platelets. We generated new canine stem cell lines that closely resemble to extraembryonic endoderm cells. These cell lines will apply to platelet production.

Free Research Field

農学