Development of procedure for single cell genomics of sedimentary microbial cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14907
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Environmental agriculture(including landscape science)
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: HOSHINO Tatsuhiko 国立研究開発法人海洋研究開発機構, 高知コア研究所, 主任研究員 (30386619)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 微生物 / DNA増幅 / 遺伝子解析

Outline of Final Research Achievements

Single cell genomics is an effective tool for understanding microbial ecology in the natural environment because it can provide comprehensive information on microbial phylogeny and metabolic function. However, application of single cell genomics to microbial cells in subseafloor environment is hampered by the fact that DNA content in a single cells is very little to be amplified to enough amount for sequencing. In this study, we developed a procedure where proteinase K is used for lysis of cells and a metal plate is used for multiple displacement amplification of genomic DNA. As a result, we successfully amplified genomic DNA from sorted single cells isolated from subseafloor sediment.

Free Research Field

環境微生物学