Use of abundant proteins as molecular recording media: Exhaustive screening strategies for chemical modifications

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14935
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: Tohoku University
• Principal Investigator: OE Tomoyuki 東北大学, 薬学研究科, 教授 (10203712)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 化学修飾 / アルブミン / ヘモグロビン / ケラチン / 分子カルテ

Outline of Final Research Achievements

Proteins are exposed to higher physiological/chemical stresses in some biological events. The resulting chemical modifications on proteins (such as oxidation, glycation, and lipidation) can provide meaningful information about the biological events. Therefore, abundant proteins (such as albumin, hemoglobin, and keratin), that are major targets of chemical modifications,can work as molecular medical record. From the view point, following results have been obtained: i) Chemical modifications on albumin has been screened and found the difference between individuals, ii) A sensitive and simultaneous analytical method has been developed for oxidized, nitrated, lipidated, and glycated hemoglobin, iii) A sensitive and non-invasive analytical method has been developed to estimate UV irradiation-induced skin damage through keratin oxidation.

Free Research Field

臨床分析化学