Basement membrane live-imaging

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15021
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Kitasato University
• Principal Investigator: Kadoya Yuichi 北里大学, 医療衛生学部, 教授 (10185887)
• Research Collaborator: Sekiguchi Kiyotoshi 大阪大学, 蛋白質研究所, 教授
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 基底膜 / ライブイメージング / ナイドゲン(nidogen) / 顎下腺 / 分枝形態形成 / EGFP

Outline of Final Research Achievements

We have recently generated recombinant human nidogen-1 whose C-terminus is fused to enhanced green fluorescent protein (hNid1-EGFP). Because nidogen-1 binds to several other major BM proteins, an attempt was made to label BM of living tissues with exogenous hNid1-EGFP and to reveal BM dynamics. Developing submandibular gland (SMG) of mouse was chosen because of its rapid expansion of BM as a result of epithelial branching. Embryonic day-13 SMG was organ cultured in the glass-bottom-dish with a medium containing hNid1-EGFP. After 15min of incubation, confocal microscopy revealed linear deposition of hNid1-EGFP sin the epithelial BMs.
We conclude that hNid1-EGFP is a unique and useful tool to study BM dynamics in vitro.

Free Research Field

解剖学（含む組織学）