Isolation and characterization of biliary tree stem cell (BTSC)

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15115
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: National Center for Global Health and Medicine
• Principal Investigator: Tanaka Minoru 国立研究開発法人国立国際医療研究センター, その他部局等, 細胞療法開発研究室長 (80321909)
• Co-Investigator(Renkei-kenkyūsha): Okochi Hitoshi 国立国際医療研究センター研究所, 細胞組織再生医学研究部, 部長 (30185235)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 胆道 / 幹細胞 / 胆管上皮細胞 / 付属線

Outline of Final Research Achievements

In this study, we demonstrated that Trop2 expression is useful to distinguish peribiliary gland (PGB)-forming epithelial cells and luminal cells in extrahepatic bile ducts. We showed the possibility that TROP2-negative PGB-forming cells could contribute to bile duct regeneration by supplying TROP2-positive transit amplifying progenitor cells by in vitro culture system, FACS analysis and immunostaining. Furthermore, we identified Samd5 as a novel PBG marker. We showed the possibility that cholangiocarcinoma can be derived from PBG based on SAMD5 expression and that SAMD5 is useful to define the different origins of intrahepatic and extrahepatic cholangiocarcinomas. SAMD5 may become a therapeutic target to treat extrahepatic cholangiocacrinomas.

Free Research Field

幹細胞生物学