2015 Fiscal Year Final Research Report
Development of highly specific oncolytic herpes virus vectors by modification of two envelope glycoproteins
| Project/Area Number |
15K15144
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Virology
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KOJIMA MASAKI 東京薬科大学, 生命科学部, 教授 (90277252)
TAHARA HIDEAKI 東京大学, 医科学研究所, 教授 (70322071)
|
|---|
| Project Period (FY) |
2015-04-01 – 2016-03-31
|
| Keywords | 遺伝子治療 / 腫瘍溶解性ウイルス療法 / ヘルペスウイルス / 抗体 / がん |
|---|
| Outline of Final Research Achievements |
Herpes simplex virus (HSV) vectors are promising agents for oncolytic virotherapy. We have recently reported a fully retargeted HSV platform that incorporates single-chain antibodies (scFv) into gD to mediate entry exclusively via tumor-associated antigens, including epidermal growth factor receptor (EGFR). In this study, we sought to examine whether another envelope glycoprotein, gB, could also be genetically re-engineered to interact with tumor-associated antigens. We inserted an anti-EGFR scFv or the EGF ligand into a number of different portions of gB, and found that many, but not all, of the mutated gB proteins were expressed on the cell surface. We also found that the viruses incorporating some of the mutated gB proteins retained the capability of entering cells, suggesting the potential feasibility of gB retargeting.
|
| Free Research Field |
遺伝子治療
|
