Explicit representation of holistic antibody repertoire by exhaustive RNA sequencing and the antibody expression by reverse genetics.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K15159
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Immunology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: OHNISHI Kazuo 国立感染症研究所, 免疫部, 主任研究官 (90169011)
• Co-Investigator(Kenkyū-buntansha): 藤 博幸 関西学院大学, 理工学部, 教授 (70192656) ; 野口 保 明治薬科大学, 薬学部, 教授 (00357740) ; 藤本 浩文 国立感染症研究所, 品質保証・管理部, 室長 (60373396)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 抗体レパトア全体像 / 抗体レパトア発生機構 / 自己抗原反応レパトア / 抗原応答抗体予測 / 抗体遺伝子合成 / 抗原抗体反応測定 / VDJ遺伝子再構成制御 / 次世代シークエンサー

Outline of Final Research Achievements

The novel system for visualization and dynamics analysis of holistic antibody repertoire space, that is generated by V(D)J-rearrangement forming the diversity of antigen recognition, by exhaustive RNA sequencing was successfully established.
The process of mouse antibody repertoire development, the response dynamics of antibody repertoire on antigen stimulation, the expression of important antibody molecules through gene-synthesis and reverse genetics, and the role of pre-B cell receptor on the repertoire formation were examined by the new system and the several important findings were obtained.
This novel analysis system is applicable for the development of antibody drugs against newly emerging infectious diseases and for planning the vaccine developments.

Free Research Field

免疫学