2016 Fiscal Year Final Research Report
Detection of circulationg tumor cells with porphyrin fluorescense
| Project/Area Number |
15K15188
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 循環腫瘍細胞 / アミノレブリン酸 / ポルフィリン |
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| Outline of Final Research Achievements |
The aims of this study are to establish a circulating tumor cells (CTCs) detection method using porphyrin fluorescence after the treatment with both aminolevulinic acid (ALA) and hemato-porphyrin (HP). Cancer cells are co-cultured with both ALA and HP, thereafter the cells are injected to mice. Spectrometric analyses of the CTCs demonstrate that cancer cells emit porphyrin fluorescence. Whole blood is analyzed by fluorescence activated cell sorter (FACS) after a hemato cellular lytic treatment to find CTCs with porphyrin fluorescence. We have reported that reactive oxygen species (ROS) involved a cancer cellular specific porphyrin fluorescence, and indomethacin (IND), a NSAID accelerated mitochondrial ROS. Thus we elucidate whether ROS induced by IND treatment accelerates cancer cellular porphyrin fluorescence. IND treatment enhances cancer cellular specific ROS to involve porphyrin fluorescence acceleration. Thus we conclude that IND pretreatment can be a clue to detect CTCs.
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| Free Research Field |
消化器病学
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