2016 Fiscal Year Final Research Report
Fine tuning the immune response through physiological hemophagocytosis
| Project/Area Number |
15K15197
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 血管新生 / 血球貪食 / 鉄 / 免疫 / リンパ管新生 / リンパ節 / B細胞 / VEGF |
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| Outline of Final Research Achievements |
Aim: Fine tuning the immune response through hemophagocytosis:Materials and methods: The CD19Cre/hVEGF-Afl mice were employed for this study. H&E staining and immunohistochemical staining were performed to identify hemophagocytosis in LNs. Prussian blue staining was done for quantification of iron deposition in tissues. The number of CD8+ T cells and programmed cell death 1 (PD-1) positive cells in LNs were analyzed by flow cytometry. Hepcidin expression in the liver was analyzed by RT-PCR. Result: The active hemophagocytosis in LNs of CD19Cre/hVEGF-Afl mice were observed together with decreasing the number of CD8+ T cells and increasing PD-1 expression in CD8+ T cells. Also CD19Cre/hVEGF-Afl mice represented the phenotype corresponding with iron deficiency anemia. Conclusion: B-cell derived VEGF-A executes tuning of immune response by decreasing the number of CD8+ T cells, increasing PD-1 expression in CD8+ T cells and hemophagocytosis.
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| Free Research Field |
血管新生、血液凝固、免疫、HIV感染症
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