Establishment of enterohormone farm by using cultured human intestinal epithelial cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15286
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Okamoto Ryuichi 東京医科歯科大学, 再生医療研究センター, 教授 (50451935)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 腸上皮 / 消化管ホルモン

Outline of Final Research Achievements

In this study, we investigated the method to induce secretory-lineage differentiation of cultured human intestinal organoids, and also performed some functional analyses of those organoids. Consequently, we successfully established over 40-lines of human small intestinal- or colonic-organoids from endoscopic biopsy specimens. Short-term exposure to a gamma-secretase inhibitor, combined with depletion of Wnt3a, SB20190 and Nicotinamide, certainly directed human intestinal organoids towards secretory cell-lineage differentiation. We also established a quantitative analysis system to evaluate the response of human intestinal organoids to a secretagogue, PGE2, or an enterohormone, VIP, and showed their properties to regulate the body-fluid balance.

Free Research Field

消化器内科学