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2016 Fiscal Year Final Research Report

Single cell imaging in human intestinal stem cell

Research Project

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Project/Area Number 15K15287
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Gastroenterology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

Tsuchiya Kiichiro  東京医科歯科大学, 医学部附属病院, 准教授 (40376786)

Project Period (FY) 2015-04-01 – 2017-03-31
Keywords1幹細胞可視化 / Lgr5プロモーター / 1幹細胞動態
Outline of Final Research Achievements

We did random labeling of fluorescence into the organoid cells by original method. We then found single fluorescence-labeled cell in crypt and isolated it. The organoids could be generated from single fluorescence cell, indicating that single fluorescence cell might be intestinal stem cell. We could therefore observe the cell division of single fluorescence stem cell and lineage tracing of differentiated cell in three dimensions. Moreover, we evaluated the role between stem cells for the formation of one villus by analyzing the mutual relations with the fluorescence-negative stem cell and fluorescence stem cell. In addition, we started the evaluation of single stem cell using human intestinal organoids to estimate the regulation of single stem cell derived from the patients.

Free Research Field

消化器内科学

URL: 

Published: 2018-03-22  

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