Cancer stem cell assay by a new immunodeficient mouse model Cancer stem cell assay by a new immunodeficient mouse model harboring with optimal bone marrow microenvironment

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K15365
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: Kyushu University
• Principal Investigator: Akashi Koichi 九州大学, 医学研究院, 教授 (80380385)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: がん幹細胞 / 異種移植アッセイ / 免疫不全マウス / マクロファージ / SIRPA / 骨髄ニッチ

Outline of Final Research Achievements

To evaluate human cancer stem cells in xenotransplantation models, a variety of immunodeficient mouse lines have been developed. We found that in xenograft engraftment, inhibition of phagocytic reaction of mouse macrophages is essential in addition to lymphoid depletion. Here, we have established a new immunodeficient BRGhS mouse line, harbouring a complete macrophage tolerance. Furthermore, we introduced KITW/v mutation into BRGhS line to optimize mouse bone marrow microenvironment for human cell engraftment. This strain should be very useful for human cancer stem cell assay.

Free Research Field

血液内科