次世代ペリオスチン測定キットの開発

2016 Fiscal Year Research-status Report

• Project/Area Number: 15K15372
• Research Institution: Saga University
• Principal Investigator: 出原 賢治 佐賀大学, 医学部, 教授 (00270463)
• Co-Investigator(Kenkyū-buntansha): 太田 昭一郎 佐賀大学, 医学部, 研究講師 (20346886) [Withdrawn] ; 有馬 和彦 佐賀大学, 医学部, 准教授 (60336112) [Withdrawn] ; 小川 雅弘 佐賀大学, 医学部, 助教 (90599317) ; 南里 康弘 (宮内康弘) 佐賀大学, 医学部, 特別研究員 (00382218) ; 布村 聡 佐賀大学, 医学部, 助教 (70424728)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: バイオマーカー / アレルギー性結膜炎 / ペリオスチン / 涙液

Outline of Annual Research Achievements

涙液ペリオスチンを測定するための迅速測定システムの構築を検討した。その結果、まず涙液を試験紙で収集し、それを測定溶液に溶解して検体として測定に用いるようにした。この方法だと、涙液の容量は5 mLで充分であった。Immunotrap法と磁気ビーズを組み合わせてペリオスチン検出システムを構築した。これにより、涙液ペリオスチンの検出は1分で可能となった。カットオフ値は10 ng/mLに設定し、その結果、健常人とアレルギー性結膜炎患者との鑑別における感度、特異度はともにほぼ1.0であった。以上より、涙液ペリオスチンを測定するための迅速測定システムの構築に成功した。現在、アレルギー性結膜炎患者の涙液を用いた臨床性能試験の実施を計画している。これにおいて満足した結果を得ることができれば、PMDAに提出して体外診断薬システムとしての認可を獲得する予定である。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

今年度涙液ペリオスチンを測定するための迅速測定システムの構築に成功した。これによりアレルギー性結膜炎患者に対する体外診断薬の開発に道筋を作ることができ、当該疾患の診療に非常に有用となると期待される。

Strategy for Future Research Activity

当初の目標通り、涙液ペリオスチンを測定するための迅速測定システムの構築に成功した。今後、ペリオスチンの体液、あるいは血清内での性状について解析を進め、新たなペリオスチン測定キットの開発につなげる。

Research Products

• [Journal Article] Reduced vitreal concentration of periostin after vitrectomy in patients with proliferative diabetic retinopathy (2016)
  • Author(s): Tachibana T, Yoshida S, Kobayashi Y, Nakama T, Ishikawa K, Nakao S, Izuhara K, Kono T, Ishibashi T
  • Journal Title: Acta Ophthalmologica Volume: 94 Pages: e81-e82
  • DOI: 10.1111/aos.12752
  • Peer Reviewed / Open Access
• [Journal Article] Increased YKL-40 and chitotriosidase in asthma and chronic obstructive pulmonary disease (2016)
  • Author(s): James AJ, Reinius LE, Verhoek M, Gomes A, Kupczyk M, Hammar U, Ono J, Ohta S, Izuhara K, Bel E, Kere J, Söderhäll C, Dahlén B, Boot RG, Dahlén S
  • Journal Title: American Journal of Respiratory and Critical Care Medicine Volume: 193 Pages: 131-142
  • DOI: 10.1164/rccm.201504-0760OC
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Roles of periostin in respiratory disorders (2016)
  • Author(s): Izuhara K, Conway SJ, Moore BB, Matsumoto H, Holweg CTJ, Matthews JG, Arron JR
  • Journal Title: American Journal of Respiratory and Critical Care Medicine Volume: 193 Pages: 949-956
  • DOI: 10.1164/rccm.201510-2032PP
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Elevation of serum squamous cell carcinoma antigen 2 in patients with psoriasis: associations with disease severity and response to the treatment (2016)
  • Author(s): Watanabe Y, Yamaguchi Y, Komitsu N, Ohta S, Azuma Y, Izuhara K, Aihara M
  • Journal Title: British Journal of Dermatology Volume: 174 Pages: 1327-1336
  • DOI: 10.1111/bjd.14426
  • Peer Reviewed / Open Access
• [Journal Article] Periostin and interleukin-13 are independently related to chronic spontaneous urticaria (2016)
  • Author(s): Bae Y, Izuhara K, Ohta S, Ono J, Hong GU, Ro JY, Park GH, Choi JH
  • Journal Title: Allergy, Asthma & Immunology Research Volume: 8 Pages: 457-460
  • DOI: 10.4168/aair.2016.8.5.457
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Multidimensional endotyping in severe asthma reveals inflammatory heterogeneity in MMPs and YKL-40 (2016)
  • Author(s): Hinks T, Brown T, Lau LCK, Rupani H, Barber C, Elliott S, Ward JA, Ono J, Ohta S, Izuhara K, Djukanovi R, Kurukulaaratchy R, Chauhan A, Howarth PH
  • Journal Title: Journal of Allergy and Clinical Immunology Volume: 138 Pages: 61-75
  • DOI: 10.1016/j.jaci.2015.11.020
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The usefulness of measuring tear periostin for the diagnosis and management of ocular allergic diseases (2016)
  • Author(s): Fujishima H, Okada N, Matsumoto K, Fukagawa K, Igarashi A, Matsuda A, Ono J, Ohta S, Mukai H, Yoshikawa M, Izuhara K
  • Journal Title: Journal of Allergy and Clinical Immunology Volume: 138 Pages: 459-467
  • DOI: 10.1016/j.jaci.2015.11.039
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Using periostin as a biomarker in the treatment of asthma (2016)
  • Author(s): Izuhara K, Ohta S, Ono S
  • Journal Title: Allergy, Asthma and Immunology Research Volume: 8 Pages: 491-498
  • DOI: 10.4168/aair.2016.8.6.491
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Allergen immunotherapy for allergic asthma: protocol for a systematic review (2016)
  • Author(s): Dhami S, Nurmatov U, Agache I, Lau S, Muraro A, Jutel M, Roberts G, Akdis C, Bonini M, Calderon M, Casale T, Cavkaytar O, Cox L, Demoly P, Flood B, Hamelmann E, Izuhara K
  • Journal Title: Clinical and Translational Allergy Volume: 6 Pages: 5
  • DOI: 10.1186/s13601-016-0094-y. eCollection 2015
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Periostin as a biomarker for diagnosis of pediatric asthma (2016)
  • Author(s): Inoue T, Akashi K, Watanabe M, Ikeda Y, Ashizuka S, Motoki T, Suzuki R, Sagara N, Yanagida N, Sato S, Ebisawa M, Ohta S, Ono J, Izuhara K, Katunuma T
  • Journal Title: Pediatric Allergy and Immunology Volume: 27 Pages: 521-526
  • DOI: 10.1111/pai.12575
  • Peer Reviewed / Open Access
• [Journal Article] Serum periostin and free IgE levels in the responsiveness to omalizumab in patients with severe asthma (2016)
  • Author(s): Tajiri T, Matsumoto H, Gon Y, Ito R, Hashimoto S, Izuhara K, Suzukawa M, Ohta K, Ono J, Ito I, Oguma T, Inoue H, Iwata T, Nagasaki T, Kanemitsu T, Niimi A, Mishima M
  • Journal Title: Allergy Volume: 71 Pages: 1472-1479
  • DOI: 10.1111/all.12922
  • Peer Reviewed / Open Access
• [Journal Article] Clinical implication of the serum periostin level for differentiating phenotypes of NSAID hypersensitivity (2016)
  • Author(s): Kim M, Yoon MK, Lee Y-S, Izuhara K, Ohta S, Ono J, Kim J-H, Ban GY, Ye Y-M, Park H-S
  • Journal Title: Allergology International Volume: 65 Pages: 492-494
  • DOI: 10.1016/j.alit.2016.04.013
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The potential for repositioning anti-thyroid agents as anti-asthma drugs (2016)
  • Author(s): Suzuki S, Ogawa M, Ohta S, Arima K, Nunomura S, Nanri Y, Mitamura Y, Yoshihara T, Nakamura Y, Yamauchi K, Chibana K, Ishii Y, Lee JJ, Aratani Y, Kakuta S, Kubo S, Iwakura Y, Yoshida H, Izuhara K
  • Journal Title: Journal of Allergy and Clinical Immunology Volume: 138 Pages: 1458-1461
  • DOI: 10.1016/j.jaci.2016.04.047
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Periostin promotes hepatic fibrosis in mice by modulating hepatic stellate cell activation via αv integrin interaction (2016)
  • Author(s): Sugiyama A, Kanno K, Nishimichi N, Ohta S, Ono J, Conway SJ, Izuhara K, Yokosaki Y, Tazuma S
  • Journal Title: Journal of Gastroenterology Volume: 51 Pages: 1161-1174
  • DOI: 10.1007/s00535-016-1206-0
  • Peer Reviewed / Open Access
• [Journal Article] Induction of airway allergic inflammation by hypothiocyanite via epithelial cells (2016)
  • Author(s): Suzuki S, Ogawa M, Ohta S, Nunomura S, Nanri Y, Shiraishi H, Mitamura Y, Yoshihara T, James J. Lee, Izuhara K
  • Journal Title: Journal of Biological Chemistry Volume: 291 Pages: 27219-27227
  • DOI: 10.1074/jbc.M116.746909
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 新規治療薬のコンパニオン診断 (2016)
  • Author(s): 出原賢治
  • Organizer: 第3回日本アレルギー学会総合アレルギー講習会
  • Place of Presentation: 横浜
  • Year and Date: 2016-12-17
  • Invited
• [Presentation] アレルギー疾患とペリオスチン (2016)
  • Author(s): 出原賢治
  • Organizer: 第65回日本アレルギー学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2016-06-19
  • Invited
• [Presentation] Allergology Internationalから観た英文論文作製の注意点とコツ (2016)
  • Author(s): 出原賢治
  • Organizer: 第65回日本アレルギー学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2016-06-17
  • Invited
• [Presentation] The potential for repositioning anti-thyroid agents as anti-asthma drugs (2016)
  • Author(s): Izuhara K, Suzuki S, Ogawa M, Ohta S, Nanri Y
  • Organizer: 31st Collegium Internationale Allergologicum
  • Place of Presentation: Charleston (USA)
  • Year and Date: 2016-04-06
  • Int'l Joint Research
• [Book] llergies: Current challenges and solutions (2016)
  • Author(s): Izuhara K, Matsumoto H, Nagasaki T, Ohta S, Ono J, Kinki Hokuriku Airway Disease Conference
  • Total Pages: 239
  • Publisher: Pacini Editore Medicina
• [Remarks] 佐賀大学医学部分子生命科学講座分子医化学ホームページ
  • URL: http://www.biomol.med.saga-u.ac.jp/medbiochem/index.php