Immune complexome analysis of tissue-deposited immune complexes for application to tissue in autoimmune diseases

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K15373
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Collagenous pathology/Allergology
• Research Institution: Nagasaki University
• Principal Investigator: OHYAMA Kaname 長崎大学, 医歯薬学総合研究科(薬学系), 准教授 (50437860)
• Co-Investigator(Kenkyū-buntansha): 川上 純 長崎大学, 医歯薬学総合研究科(医学系), 教授 (90325639) ; 中村 英樹 長崎大学, 病院(医学系), 講師 (10437832)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 免疫複合体

Outline of Final Research Achievements

We have developed "immune complexome analysis", in which ICs are separated from whole serum and then subjected to direct tryptic digestion and nano-liquid chromatography-tandem mass spectrometry. We have successfully used this method to identify disease-specific IC antigens in the sera of patients with autoimmune diseases. In this research, we aimed to develop "comprehensive analysis method of tissue-deposited immune complexes" by applying this analysis method. As a result of the optimization, it was found that reconstitution after extracting, which is less efficient than anticipated, is not advanced in antigen stimulation of metamorphic antigens in tissues. In the future, we will analyze tissue analysis of immune complexes using surface MS analysis such as liquid extraction surface analysis.

Free Research Field

臨床化学