2016 Fiscal Year Final Research Report
Elucidation of temporo-spatial heterogeneity of glioma by Liquid Biopsy
| Project/Area Number |
15K15529
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurosurgery
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | glioma / liquid biopsy / cell free DNA / microRNA / digital PCR |
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| Outline of Final Research Achievements |
We started Liquid Biopsy by circulating cell free DNA (cfDNA) and microRNA (miRNA) analysis in body fluids for glioma cases. Firstly, global expression analysis of blood miRNA revealed that expression of many miRNA is elevated in glioma cases, and some specific miRNAs could be analyzed also in cerebrospinal fluid. Secondly, cfDNA analysis was undertaken. First, mutation analysis of IDH1 was carried out using the TaqMan method, but due to the small amount of cfDNA, it was difficult to accurately evaluate the mutation, and most of the cases were not analyzable. Next, we introduced digital PCR, and it was clarified that mutation analysis of IDH1 gene is possible by analysis of cfDNA in cerebrospinal fluid.
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| Free Research Field |
neurooncology
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