Development of novel target therapy for metastatic organ of malignant soft tissue tumor

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K15560
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: 医療法人徳洲会野崎徳洲会病院(附属研究所) (2016-2017); Research Institute, Osaka Medical Center for Cancer and Cardiovascular Disaeses (2015)
• Principal Investigator: Itoh Kazuyuki 医療法人徳洲会野崎徳洲会病院(附属研究所), 研究所, 所長 (20301806)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 肺転移 / スフェロイド

Outline of Final Research Achievements

Prognosis of malignant soft tissue tumor clinically depended on the lung metastasis. Recently, critical roles of spheroid formation and dynamics during metastasis formation was emphasized. We herein newly developed macro-microscopy with near-infrared light. Employing this system, we could analyze the marginal zones and also intra regions of malignant soft tissue tumor spheroid with time-lapse recording.
Numerous filopodias were observed surrounding spheroid with Cdc42-dependent fashion and budding (
sprouting) formation were observed occasionally with Rho-Rho-kinase (ROCK) dependent manner. We are currently analyzing this spheroid dynamics with several compounds, which already confirmed showing the inhibitory effects for the same soft tissue tumor in vitro and also in vivo Xenograft model.

Free Research Field

腫瘍生物学