Investigation of effects of anesthetics on phenotype and gene expression of cancer cells with CTOS method

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15577
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Anesthesiology
• Research Institution: Kansai Medical University
• Principal Investigator: IWAI Teppai 関西医科大学, 医学部, 助教 (90440966)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 麻酔薬 / がん細胞 / 悪性度 / CTOS / HIF-1 / RNA-Seq

Outline of Final Research Achievements

Growing evidence indicates that perioperative factors, including choice of anesthetic, affect cancer recurrence after surgery although little is known about the effect of anesthetics on cancer cells themselves. Certain anesthetics are known to affect phenotypes of cells derived from cancer. In this study, we used cells established from cancer tissue and the primary culture cells from the cancer tissue-originated spheroid (CTOS) method.
Our results provide evidence that the anesthetics isoflurane and sevoflurane did not exert a pro-tumorigenic effect on human cancer cell lines and spheroid cells by CTOS. We present evidence that the anesthetics did not affect phenotypes of cells derived from cancer, cell signaling pathway involving in tumorigenesis nor gene expression patterns associated with a malignant phenotype.

Free Research Field

麻酔科学