2016 Fiscal Year Final Research Report
Elucidating the mechanism of resistance to chemotherapy in gynecologic cancer based on intratumor heterogeneity
| Project/Area Number |
15K15598
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Niigata University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | intratumor heterogeneity / cancer genome / spheroid |
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| Outline of Final Research Achievements |
In this study, we generated total 11 spheroid cultures derived from gynecologic cancers. Before genomic analyses for spheroid cultures, we focused on Sex-determining region Y-box 2 (SOX2), which is an essential factor involved in the self-renewal and pluripotency of embryonic stem cells, to clarify the significance of SOX2 expression in endometrial cancer. SOX2 overexpression regulated the expression of cyclin-dependent kinase inhibitor 1A (CDKN1A), and SOX2 specifically bound to p21 promoter DNA in endometrial cancer cell lines expressing SOX2. Expressions of SOX2 in endometrial cancer patients were significantly correlated with histological grade and poor prognosis. Moreover, low p21 together with high SOX2 expressions in advanced endometrial cancer patients were associated with the most unfavorable outcomes of patients. These results indicated that simultaneous measurement of SOX2 and p21 expression in endometrial cancer patients may be a useful biomarker for patient prognosis.
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| Free Research Field |
婦人科腫瘍
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