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2016 Fiscal Year Final Research Report

Challenges for effective delivery of stem cells to the organ of Corti

Research Project

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Project/Area Number 15K15615
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Otorhinolaryngology
Research InstitutionYamagata University

Principal Investigator

Kakehata Seiji  山形大学, 医学部, 教授 (90261619)

Project Period (FY) 2015-04-01 – 2017-03-31
Keywords内耳障害 / 難聴 / 幹細胞移植
Outline of Final Research Achievements

Approximately 250-350g, male Hartley strain guinea pigs were exposed to 4-8 kHz octave-band noise at 136 dB sound pressure level (SPL) for 2 hours in the sound proof box under general anesthesia. All the animals were measured their ABR threshold before and 24 hours, 1 week and 2 weeks after the noise exposure to confirm the permanent threshold shift. The ABR threshold of 2 weeks after the noise exposure showed profound deafness. Cochlear frequency mapping was performed with ImageJ plugin to identify the tonotopic area, demonstrating that outer hair cells were disappeared.
Multilineage-differentiating stress-enduring (Muse) cells were transplanted into the Scala tympani of the severely damaged animals of the number of 10,000 or 30,000 cells through a micro catheter. ABR of the transfected ears did not show evident hearing improvement compared to the control group. Immunohistochemistrical data show no GFP-positive cells alive in the cochlear.

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Published: 2018-03-22  

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