2016 Fiscal Year Final Research Report
Analysis of molecular basis of retinal glial cells and their roles in retinal degeneration diseases
| Project/Area Number |
15K15628
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Keywords | 網膜 / ミュラーグリア / マイクログリア / 変性 / 遺伝子発現 / ヒストンメチル化 |
|---|
| Outline of Final Research Achievements |
We established technic to purify photoreceptor, Mueller glia, and microglia by using multiple cell surface antigens and a cell sorter. Then, these retinal subsets were purified from normal and photoreceptor-degenerated retina, and RNA-seq was performed using the fractions. Genes specifically up-regulated in each retinal subtype were identified by bio-informatics technics. Time course of gene expression was examined, and interaction of the subtypes through cytokines and chemokines during retinal degeneration was examined from the results.
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| Free Research Field |
分子生物学 眼科学
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