Establishment of a live cell imaging system for the central retina by two-photon microscopy

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15631
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Nagoya University
• Principal Investigator: Osakada Fumitaka 名古屋大学, 大学院創薬科学研究科, 准教授 (60455334)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 2光子顕微鏡 / 視細胞 / クロマチン / 発生 / 再生 / ライブイメージング / 網膜 / 細胞核

Outline of Final Research Achievements

Regenerative medicine represents a promising future treatment of retinal degenerative diseases by making use of the proliferation and differentiation potential of stem cells. At present, transplantation of photoreceptors in the peripheral retina can be most promising therapy. Regeneration of the central retina, however, has not been tried due to the lack of appropriate methods that evaluate the function of the central retina with cellular resolution in mice and monkeys. In the present study, we succeeded in establishment of two-photon microscope system with imaging chamber that makes it possible to evaluate the development and function of photoreceptors in the live retina.

Free Research Field

眼科学