Regeneration of hypoplastic lung with congenital diaphragmatic hernia using by umbilical cord mesenchymal stem cell sheet

2018 Fiscal Year Final Research Report

• Project/Area Number: 15K15642
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pediatric surgery
• Research Institution: University of Tsukuba
• Principal Investigator: Shinkai Toko 筑波大学, 医学医療系, 講師 (80301612)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Keywords: 再生医療 / 臍帯由来間葉系幹細胞 / 横隔膜ヘルニア / 肺低形成

Outline of Final Research Achievements

We observed the differentiation induction of fetal rat lung by co-culture with umbilical cord-derived mesenchymal stem cells (UC-WJSCs) and fetal rat lung which suitable for observation. We compared the usefulness of UC-WJSCs by the difference between Lung bud increase rate and expression of mRNA involved in fetal lung growth. The lung bud increment was significantly high in the co-culture group with UC-WJSCs. In addition, the mRNA expressions of VEGF, BMP-4 and TTF-1, which are factors of lung angiogenesis and lung branching morphogenesis, were significantly higher in the co-culture group with UC-WJSCs. However, there was no significant difference between the two groups in the expression of Shh.

Free Research Field

小児外科

Academic Significance and Societal Importance of the Research Achievements

本研究は小児の難治性疾患に対する再生医療の可能性を示す基礎実験となったと考えられる。また、出産後に廃棄される臍帯を再生医療の原資に用いる可能性を示せたことも本研究の学術的、社会的な意義は大きいものと考える。