Diaphragmatic regeneration using umbilical cord blood derived stem cels for diaphragmatic agenesis

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K15646
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pediatric surgery
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Tajiri Tatsuro 京都府立医科大学, 医学(系)研究科(研究院), 教授 (80304806)
• Co-Investigator(Kenkyū-buntansha): 岸田 綱郎 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (00370205) ; 木村 修 京都府立医科大学, 医学(系)研究科(研究院), 特任教授 (10315963) ; 東 真弓 京都府立医科大学, 医学(系)研究科(研究院), 助教 (10380453) ; 文野 誠久 京都府立医科大学, 医学(系)研究科(研究院), 助手 (40405254)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 再生医学 / 小児外科 / 先天性横隔膜ヘルニア / 骨格筋 / 臍帯血

Outline of Final Research Achievements

Diaphragmatic regeneration using umbilical cord blood derived stem cels for diaphragmatic agenesis was attempted using 3 types of different stem cells. Connection of small muscle sphere was not observed. Therefore, we attempted direct conversion from human fibroblast into myoblast using MyoD1 and L-myc gene trasfection as next step. Finally, we obtained
multinucleated muscle cells with high efficiency. we perspective function myosheet using this novel technique.

Free Research Field

医歯薬学