Elucidation of Mg2+-metabolic mechanisms through the Channel-kinase TRPM7

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K15689
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Functional basic dentistry
• Research Institution: Fukuoka Dental College
• Principal Investigator: okabe koji 福岡歯科大学, 口腔歯学部, 教授 (80224046)
• Co-Investigator(Kenkyū-buntansha): 岡本 富士雄 福岡歯科大学, 口腔歯学部, 講師 (60153938) ; 福島 秀文 東北大学, 歯学研究科, 准教授 (70412624)
• Co-Investigator(Renkei-kenkyūsha): MATSUSHITA Masayuki 琉球大学, 医学(系)研究科, 教授 (30273965)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: TRPM7 / 破骨細胞 / コンディショナルKOマウス / Mg2+代謝機構

Outline of Final Research Achievements

We investigated Mg2+-metabolic mechanisms through the Channel-kinase TRPM7 which is a Mg2+-permeable cation channel in osteoclasts. Analyses of bone morphometry and serum Mg2+ concentration were performed using the osteoclast-specific TRPM7 conditional KO (cKO) mice and TRPM7-kinase mutant (KR) mice. There were no significant differences in bone morphometry parameters, serum Mg2+ concentration and bone resorption activity of cultivated osteoclast between cKO and KR and wild type mice of 8-week-old. On the other hand, 30-week-old cKO mice showed a significant increase in bone volume and decrease in the number of osteoclast. These findings suggest that Mg2+-permeable TRPM7 channel plays an important role in the bone resorption and bone remodeling by osteoclasts with aging.

Free Research Field

生理学