2017 Fiscal Year Final Research Report
Vitamin D regulates dental epithelial cell differentiation and enamel formation
| Project/Area Number |
15K15752
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | エナメル / ビタミンD / ビタミンＤ欠乏症 / 25OHD3 / 歯原性上皮細胞 / 紫外線 / 1,25(OH)2D3 |
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| Outline of Final Research Achievements |
The aim of this research is to elucidate the mechanism of vitamin D during enamel formation as well as enamel mineralization. 1,25(OH)2D3 promoted the expression of a series of enamel matrix proteins such as Ameloblastin, enamelin, and amelogenin in dental epithelial cells. Thus, 1,25(OH)2D3 promotes dental epithelial cell differentiation into ameloblast. In addition, long term culture of dental epithelial cells in the presence of 10-7M 1,25(OH)2D3 with mineralization media induced the mineralization of culture cells as well as secreted matrices. Attrition of the tip of incisors and molars are observed in rats which feed vitamin D-deficient diet for 12 weeks. Furthermore, incisors of vitamin D-deficient rats showed chalky white enamel suggesting enamel hypomineralization. These results suggest that 1,25(OH)2D3 is an essential vitamin for normal enamel formation.
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| Free Research Field |
歯科薬理学
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