Analysis of radiation induced mutation patterns and frequencies with whole genome sequencing

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K16125
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Hiroshima University
• Principal Investigator: Kanai Akinori 広島大学, 原爆放射線医科学研究所, 助教 (60549567)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 放射線 / 全ゲノム解析

Outline of Final Research Achievements

To estimate radiation induced mutation patterns and frequencies with whole genome sequencing, HT1080 single cells were gamma-irradiated 84 mGy, 390 mGy and 1885 mGy. The cells were cultured, DNA was extracted and we performed whole genome sequencing using HiSeq2500. Large deletions were rarely detected and there was no difference with dose dependent. The numbers of snps, small insertions and deletions were increased in 1885 mGy irradiated cell.

Free Research Field

分子生物学