2018 Fiscal Year Final Research Report

Establishment of a system for screening food factors inhibiting the absorption of Cs in intestine

Research Project

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Project/Area Number	15K16211
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Eating habits
Research Institution	Sugiyama Jogakuen University (2017-2018) University of Shizuoka (2015-2016)
Principal Investigator	Yasuda Michiko 椙山女学園大学, 生活科学部, 講師 (00707036)
Research Collaborator	Shimoi Kayoko Karaki Shin-ichiro Masuda Syuichi
Project Period (FY)	2015-04-01 – 2019-03-31
Keywords	セシウム / 腸管 / Caco-2細胞 / 食品機能 / ケルセチン / ゲニステイン / グルカン
Outline of Final Research Achievements	The aim of this study was the establishment of a system for screening food factors inhibiting the absorption of Cs in intestine and the investigation of food factors using Caco-2 cell system in tissue culture insert, a well characterized intestinal in vitro model, and the Ussing chamber system equipped with intestinal tissue from C57BL/6 mice. CsCl solution was added to apical side in Caco-2 system. After incubation for various lengths of time, aliquots of the buffer in opposite chambers are removed, and digested with a microwave digestion procedure for the determination of Cs concentration by ICP-MS. In Ussing chamber system with mouse jejunum, CsCl solution (1.0 mM) was added to mucosal side, and aliquots of the buffer in opposite chambers are corrected after incubation and digested as above. In summary, we developed the system for screening food factors inhibiting the absorption of Cs in intestine, and investigated the effects of food flavonoids on these evaluation systems.
Free Research Field	食品機能学
Academic Significance and Societal Importance of the Research Achievements	摂取することで放射性セシウムによる内部被ばくを防ぐ薬剤のプルシアンブルーとリンゴのペクチンには研究成果報告に示したように問題点がある。本研究により、まず、これらの問題点を解決するための食品成分に関する検討が可能になる。また、これを使って、実際にセシウムの腸管での移行を調節する食品成分を得られた場合、ヒトへの内部被ばく対策として用いることができると共に、食用動物等にも応用できる可能性がある。また、風評被害を含め、放射性物質による事故の際の食材に対する風評被害も含めた、人々への不安軽減にも役立つことが考えられる。