Investigation for action mechanism of licorice functional flavonoids using a monoclonal antibody.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K16225
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Eating habits
• Research Institution: Nagasaki International University
• Principal Investigator: Fujii Shunsuke (藤井俊輔) 長崎国際大学, 健康栄養学科, 助教 (10610165)
• Research Collaborator: Shoyama Ykihiro 長崎国際大学, 薬学部, 名誉教授 (70037604) ; Morinaga Osamu 第一薬科大学, 薬学部, 准教授 (60465771) ; Uto Takuhiro 長崎国際大学, 薬学部, 准教授 (90469396)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: モノクローナル抗体 / 甘草 / 甘草フラボノイド / リクイリチン / グリチルリチン / グリチルレチン酸 / 3T3-L1

Outline of Final Research Achievements

We attempted to analyze for action mechanism of licorice components using monoclonal antibody as research foundation. The 3T3-L1 cell were treated to screen for inhibition of differentiation to adipocytes using various licorice flavonoids and then it was also investigated the synergistic effect with glycyrrhizin (GL) and glycyrrhetinic acid (GA). Moreover, the anti-GA mAb were prepared and developed to enzyme-linked immunosorbent assay which can be quantitative analysis. In addition, the eastern blotting technique was achieved for GL and licorice major flavonoid, liquiritin (Liq) which can detect visually using anti-GL and anti-Liq mAbs.

Free Research Field

食品化学、天然物化学