Generation of beta-cell graft using somatic reprogramming and cell fiber technology

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K16319
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: The University of Tokyo
• Principal Investigator: Nagata Shogo 東京大学, 生産技術研究所, 特任研究員 (40751441)
• Research Collaborator: TAKEUCHI Shoji 東京大学, 生産技術研究所, 教授 (90343110)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 細胞ファイバー / 三次元培養 / 移植 / 再生医療

Outline of Final Research Achievements

I researched on cell culture / transplant integrated device using cell fiber technology. The culture conditions and characteristics of cells encapsulated in the fibers were evaluated in order to apply the core-shell microfiber technology for transplantable graft.
A novel three dimensional (3D) culture system of human fibroblasts and iPS cells was established by the core-shell microfibers, in which those cells could survive and expand within the fibrous core regions. We showed that it was possible for induction of rejuvenation especially in fibroblasts by optimizing the microenvironment in the 3D space. Furthermore, it was clarified that cell fate control is possible within the fiber by culturing the cell fiber in a specific differentiation induction medium.

Free Research Field

幹細胞生物学